Plasmid for producing human proinsulin

Details Plasmid for producing human proinsulin Plasmid for producing human proinsulin

C - Chemistry, Metallurgy

12

N

195/1.2, 195/1.3

C12N 15/00 (2006.01) C07K 14/62 (2006.01) C12N 1/20 (2006.01) C12N 15/62 (2006.01) C12P 21/02 (2006.01)

Patent

CA 1201074

PLASMID FOR PRODUCING HUMAN INSULIN ABSTRACT Recombinant plasmids are described that carry the structural gene for human proinsulin in expressible form. The human proinsulin structural gene is fused to a functional transport sequence. Host organisms trans- formed by the recombinant plasmids express a preploinsulin product that is correctly processed to human proinsulin in vivo, and is transported across the host organism's cell wall. Following its recovery, in vitro methods are used to convert the human proinsulin to human insulin.

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