Plasmid for production of membrane protein, bacterium...

Details Plasmid for production of membrane protein, bacterium... Plasmid for production of membrane protein, bacterium...

C - Chemistry, Metallurgy

12

N

167/37, 530/15.0

C12N 15/31 (2006.01) A61K 39/102 (2006.01) A61K 39/40 (2006.01) A61P 31/04 (2006.01) C07K 14/285 (2006.01) C07K 16/12 (2006.01) C12N 1/21 (2006.01) C12N 5/18 (2006.01) C12N 15/70 (2006.01) C12P 21/08 (2006.01) C12Q 1/68 (2006.01) G01N 33/569 (2006.01)

Patent

CA 1341574

A plasmid which contains a genetic code for an immunogenic portion which is conserved in many strains of nontypable Haemophilus influenzae and the bacterium containing this plasmid is disclosed. The immunogenic portion is preferably an epitope on an outer membrane protein of H. influenzae. A monoclonal antibody to the immunogenic portion and the hybridoma which will produce the monoclonal antibody is also included. The invention further includes a DNA probe constructed to correspond to the nucleic acids which code for the immunogenic portion. This probe may be labelled with a radioactive marker and may be used as a diagnostic tool to assay various clinical samples for the presence of H. influenzae.

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