C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/68 (2006.01) A61K 35/74 (2006.01) C12N 15/64 (2006.01)
Patent
CA 2546775
The invention relates to a system for stable maintenance of a plasmid, to host cells for use in this system and to methods of using the system to obtain a plasmid useful in medical applications. In particular, the invention provides transformed host cell containing: i) a chromosomal gene which inhibits cell growth; and ii) a plasmid encoding an antisense sequence, wherein the antisense sequence encoded by the plasmid inhibits the action of the chromosomal gene, thereby permitting cell growth and a method for stable maintenance of a plasmid in a host cell in vivo.
L'invention concerne un système servant à maintenir un plasmide de manière stable, des cellules hôtes destinées à être utilisées dans ce système, et des procédés d'utilisation dudit système pour obtenir un plasmide pouvant être utilisé pour des applications médicales. La présente invention concerne notamment un procédé pour maintenir un plasmide de manière stable dans une cellule hôtein vivo.
Cobra Biologics Limited
Kirby Eades Gale Baker
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