Plasmid schuttle vectors conferring spiramycin resistance in...

Details Plasmid schuttle vectors conferring spiramycin resistance in... Plasmid schuttle vectors conferring spiramycin resistance in...

C - Chemistry, Metallurgy

12

N

195/1.17, 195/1.

C12N 1/20 (2006.01) C12N 15/65 (2006.01) C12N 15/76 (2006.01)

Patent

CA 1297812

X-6850 Abstract A novel gene conferring resistance to spira- mycin in Streptomyces griseofuscus was cloned from a genomic library of Streptomyces ambofaciens DNA. An eighteen kb fragment of S. ambofaciens DNA surrounding the spiramycin resistance gene was isolated from this library on a plasmid cosmid designated pKC514. The novel spiramycin-resistance gene can be isolated on an ~3.4 kb Sau3AI fragment by subcloning restriction fragments obtained from the pKC514 insert DNA. This Sau3AI fragment contains all of the information required for the expression of the spiramycin resistant phenotype in Streptomyces. Vectors and transformants containing the novel spiramycin resistance gene are provided.

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