C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/70 (2006.01)
Patent
CA 2405326
A technique of efficiently and artificially recombining a specific gene on the chromosome of a microorganism belonging to the genus Escherichia which is highly useful as a bacterium producing various useful substances. A plasmid, which is replicable in Escherichia coli K-12 strain but non replicable in microorganisms belonging to the genus Escherichia other than the Escherichia coli K-12 strain, is constructed. A target gene is inserted into this plasmid and then transferred to a microorganism belonging to the genus Escherichia other than the Escherichia coli K-12 strain. Thus, a strain having the substitution of the target gene can be efficiently selected.
La présente invention concerne une technique permettant de recombiner artificiellement et de façon puissance un gène spécifique du chromosome d'un micro-organisme appartenant au genre Escherichia qui convient particulièrement comme bactérie pour la production de diverses substances. En l'occurrence, on a construit un plasmide qui se réplique chez la souche K-12 d'Escherichia coli, mais ne peut pas se répliquer dans des micro-organismes appartenant au genre Escherichia autres que la souche K-12 d'Escherichia coli. A cet effet, on insert dans ce plasmide un gène cible et on le transfère à un micro-organisme appartenant au genre Escherichia autre que la souche K-12 d'Escherichia coli. On arrive ainsi à sélectionner de façon puissante une souche porteuse du gène cible.
Furukawa Satoru
Kino Kuniki
Takano Junichi
Goudreau Gage Dubuc
Kyowa Hakko Kogyo Co. Ltd.
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