Plasmids for plant transformation and method for using the same

Details Plasmids for plant transformation and method for using the same Plasmids for plant transformation and method for using the same

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2294703

This invention relates to the Agrobacterium mediated plant transformation, especially to transformation of plants with T-DNA, where read-through at the borders is prohibited. This can be done by inhibition of transfer of vector DNA to the plant cell by creating DNA binding sites outside the T-borders, but also to insert a coding sequence outside the T-borders which, whether or not in cooperation with genes from the host plant or genes cotransferred, are toxic to plants so that there is counterselection for plants with superfluous vector-DNA.

L'invention concerne la transformation de plantes favorisée par Agrobacterium et, en particulier, la transformation de plantes au moyen d'ADNt, ce qui interdit la poursuite de la lecture au niveau des limites. On obtient ce résultat par inhibition du transfert de l'ADN vectoriel vers la cellule de la plante, ce qui consiste à créer des sites de fixation d'ADN hors des limites T, mais également à insérer une séquence codante hors de ces limites T, qui, en coopération ou non avec des gènes provenant de la plante hôte ou des gènes co-transférés, sont toxiques pour les plantes, de sorte qu'une contre-sélection s'effectue pour les plantes comportant un ADN vectoriel superflu.

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