Plasmids from corynebacterium glutamicum and use thereof

Details Plasmids from corynebacterium glutamicum and use thereof Plasmids from corynebacterium glutamicum and use thereof

C - Chemistry, Metallurgy

12

N

C12N 1/20 (2006.01) C12N 15/10 (2006.01) C12N 15/63 (2006.01) C12N 15/77 (2006.01) C12P 13/02 (2006.01) C12P 13/04 (2006.01) C12P 13/08 (2006.01)

Patent

CA 2322657

This invention relates to the mutually compatible plasmids pTET3 and pCRY4, isolated from the strain of Corynebacterium glutamicum deposited under DSM number 5616, wherein plasmid pTET3 is characterised by 1.1 a length of ~ 27.8 kbp and the restriction map shown in Figure 1, 1.2 a replication region comprising the nucleotide sequence shown in SEQ ID no. 1 and 1.3 an antibiotic resistance region consisting of a tetA gene imparting tetracycline resistance and an aadA gene imparting streptomycin and spectinomycin resistance, shown in SEQ ID no. 6, and plasmid pCRY4 is characterised by 1.4 a length of ~ 48 kbp and the restriction map shown in Figure 2 and 1.5 a replication region comprising the nucleotide sequence shown in SEQ ID no. 4 to composite plasmid vectors of these plasmids which are capable of autonomous replication in coryneform bacteria and to processes for the production of L-amino acids, vitamins and nucleotides using these bacteria.

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