Plasmids, methods for construction of the same,...

Details Plasmids, methods for construction of the same,... Plasmids, methods for construction of the same,...

C - Chemistry, Metallurgy

12

N

195/1.14, 195/1.

C12N 15/00 (2006.01) C12N 1/20 (2006.01) C12N 1/38 (2006.01) C12N 9/24 (2006.01) C12N 9/86 (2006.01) C12N 15/70 (2006.01)

Patent

CA 1226833

Specification Title of the Invention Plasmids, Methods for Construction of the same, Microorganisms Carrying the Plasmids and Methods for Cultivation of the Microorganism Abstract of the Disclosure Novel plasmids pEAP2 and pCX311, which were constructed from Bacillus sp. chromosomal DNA carrying the gene for extracellular production of such high-molecular substances as penicillinase and xylanase, and a vector plasmid pMB9 or pBR322. A novel microorganism, Escherichia coli HB101 carrying the plasmid pEAP2 [Escherichia coli HB101 (pEAP2)] and being capable of extracellular production of penicillinase. A novel microorganism, Escherichia coli HB101 carrying the plasmid pCX311 [Excherichia coli HB101 (pCX311)] and being capable of extracellular production of xylanase. Methods for cultivation of the microorganisms characterized by culturing them in the medium containing NaCl (or KCl), or NaCl (or KCl) and bran, or NaCl (or KCl) and xylan for 12-48 hours.

449095

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