C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/53 (2006.01) C07K 14/81 (2006.01) C12N 5/10 (2006.01) C12N 9/02 (2006.01) C12N 9/72 (2006.01) C12N 15/85 (2006.01)
Patent
CA 2042005
ABSTRACT OF THE DISCLOSURE There is provided a novel plasmid in which (i) a promoter capable of regulating the expression of a foreign protein in an animal cell and (ii) a DNA encoding dihydro- folate reductase (DHFR) upstream of which an urokinase (UK) promoter is ligated are incorporated. The plasmid may also be designed to incorporate therein (i) a DNA encoding a foreign protein upstream of which a promoter capable of regulating the expression of the protein an animal cell is ligated and (ii) a DNA encoding dihydrofolate reductase (DHFR) upstream of which an urokinase (UK) promoter is ligated. These plasmids are useful for the mass production of a foreign protein such as plasminogen activator, in an animal ceil by genetic engineering.
Amatsuji Yasuo
Asakura Eiji
Hayasuke Naofumi
Hirose Masaaki
Kamiya Toshio
Amatsuji Yasuo
Asakura Eiji
Fetherstonhaugh & Co.
Hayasuke Naofumi
Hirose Masaaki
LandOfFree
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