Plastid inner envelope membrane targeting polypeptides:...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/62 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01) C12N 15/29 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2229744

A polypeptide amino acid sequence capable of targeting the plastid inner envelope membrane of a plant, and a nucleotide sequence therefor, are described, as well as a chimaeric gene comprising a gene promoter, a nucleotide sequence encoding a polypeptide capable of targeting the plastid inner envelope membrane of a plant, or a variant, derivative or homologue thereof, a coding sequence and a terminator sequence. A method of transforming plants, such as potato and tobacco, using the targeting sequence so as to increase starch production is also described. The targeting sequence can be used in many other applications.

L'invention a pour objet une séquence d'acides aminés de polypeptides permettant de cibler la membrane de l'enveloppe interne du plaste d'une plante, et une séquences de nucléotides. L'invention concerne également un gène chimérique comprenant un promoteur de gènes, une séquence de nucléotides codant un polypeptide capable de cibler la membrane de l'enveloppe interne du plaste d'une plante, ou un variant, un dérivé ou un homologue de ce dernier, une séquence de codage et une séquence de terminateurs. L'invention traite aussi d'un procédé de transformation de plantes, telles que les pommes de terre et le tabac, à l'aide de la séquence de ciblage pour augmenter la production d'amidon. La séquence de ciblage peut être utilisée dans bon nombre d'autres applications.

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