C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/82 (2006.01)
Patent
CA 2356458
A method is provided for transforming Brassica plants to express DNA sequences of interest from the plant cell plastid. The method allows the transformation of Brassica plant tissue with heterologous DNA constructs. Such DNA constructs comprise, in the 5' to 3' direction of transcription, a promoter region functional in a plant plastid and a DNA sequence of interest. The invention further provides for Brassica cells in which the plastids contain heterologous DNA constructs.
L'invention concerne un procédé permettant de transformer des Brassica afin d'exprimer des séquences d'ADN d'intérêt provenant du plaste cellulaire desdites plantes. Ce procédé permet de transformer des tissus de Brassica au moyen de constructions d'ADN hétérologues. Ces constructions d'ADN comprennent, dans la direction 5'à 3' de la transcription, une zone de promoteur fonctionnelle dans un plaste, et une séquence d'ADN d'intérêt. L'invention concerne également des cellules de Brassica dans lesquelles les plastes contiennent des constructions d'ADN hétérologues.
Chaudhuri Sumita
Oakes Janette V.
Calgene Llc
Mcfadden Fincham
Monsanto Company
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