Plastid transformation of solanaceous plants

Details Plastid transformation of solanaceous plants Plastid transformation of solanaceous plants

C - Chemistry, Metallurgy

12

N

C12N 5/10 (2006.01) A01H 5/00 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2352383

A method is provided for transforming solanaceous plants to express DNA sequences interest from the plant cell plastid. The improved method allows the transformation of solanaceous plant tissue which is not obtained from tobacco with DNA constructs. Such DNA constructs comprise, in the 5' to 3' direction of transcription, a promoter region functional in a plant plastid and a DNA sequence of interest. The method can be utilized in the transformation of solanaceous plants, such as potato and petunia. The invention further provides constructs and methods for the expression of green fluorescent protein from the plant cell plastid.

L'invention concerne une méthode permettant de transformer des solanacées afin d'exprimer des séquences d'ADN d'intérêt provenant du plaste cellulaire desdites plantes. Cette méthode améliorée permet la transformation de tissus végétaux des solanacées au moyen de constructions d'ADN, à partir de plantes autres que le tabac. Ces constructions d'ADN comprennent, dans la direction 5'-3' de la transcription, une zone de promoteur fonctionnelle dans un plaste, et une séquence d'ADN d'intérêt. Cette méthode peut être utilisée pour la transformation des solanacées, telles que la pomme de terre ou le pétunia. L'invention concerne également des constructions et des méthodes destinées à l'expression de la protéine GFP à partir du plaste cellulaire végétal.

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