C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/0735 (2010.01) C12N 5/073 (2010.01) A01K 67/027 (2006.01) C12N 5/10 (2006.01)
Patent
CA 2311396
The present invention provides an isolated population of non-mouse embryonic stem (ES) cells and methods of obtaining these ES cells. In one aspect, the target ES cells are obtained by co-culturing embryo cells from a target animal with non-target ES cells, such as mouse ES cells. In one embodiment, rat ES cells are isolated from the co-culture using positive or negative selectable markers. The invention also includes genetically modified non-mouse ES cells. Chimeric embryos and animals containing isolated populations of the ES cells or genetically modified ES cells are also provided. In one embodiment, the genetic modification comprises introduction of a transgene. In another embodiment, the genetic modification comprises disruption of the function of one or more genes.
La présente invention concerne une population isolée de cellules souches embryonnaires (ES) non murines ainsi que des procédés permettant d'obtenir ces cellules ES. Selon un aspect de l'invention, les cellules ES cibles s'obtiennent par co-culture de cellules d'embryons issues d'un animal cible et de cellules ES non cibles, telles que des cellules ES murines. Selon un mode de réalisation, les cellules ES murines sont isolées de la co-culture au moyen de marqueurs sélectionnables positifs ou négatifs. L'invention concerne également des cellules ES non murines génétiquement modifiées, ainsi que des embryons et des animaux chimères contenant des populations isolées de cellules ES ou de cellules ES génétiquement modifiées. Selon un mode de réalisation, la modification génétique comprend l'introduction d'un transgène. Selon un autre mode de réalisation, la modification génétique comprend le dérèglement de la fonction de un ou plusieurs gènes.
Arc Genomic Research
Borden Ladner Gervais Llp
LandOfFree
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