C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/073 (2010.01) C12N 5/071 (2010.01) C12N 5/0735 (2010.01) C12N 5/0789 (2010.01)
Patent
CA 2656175
The present invention relates to the field of pluripotent stem cell culture media and to methods for culturing cells to produce such media-. Furthermore, the present invention provides methods and materials for propagating pluripotent stem cells in a substantially undifferentiated state, with and without a feeder layer. Conditioned medium for the propagation of pluripotent stem cells is produced by a method comprising the steps of : a. Culturing cells that supply the conditioning factors, e.g. amniotic fluid cells or cells isolated from umbilical cord tissue, b. Adding a base medium to the cells that supply the conditioning factors, c. Exposing the base medium to the cells that supply the conditioning -factors for a period of time sufficient to condition the medium, and d. Removing the conditioned medium.
Dans le domaine de la culture de cellules souches pluripotentes et de procédés de culture de cellules en vue de la production de milieux conditionnés et de cellules nourricières, les procédés et les matériaux proposés permettent la multiplication de cellules nourricières en vue de la multiplication de cellules souches pluripotentes à l'état essentiellement indifférencié, en association ou pas avec une couche de cellules nourricières.
Fryer Benjamin
Pellegrino-Gensey J. Lee
Lifescan Inc.
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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