C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/0735 (2010.01) C12N 5/071 (2010.01) C12N 11/00 (2006.01) C12N 15/09 (2006.01) C12N 15/87 (2006.01) C07K 14/705 (2006.01)
Patent
CA 2560581
A novel method of proliferating pluripotent stem cells, such as ES cells. There are provided methods of proliferation and gene introduction for pluripotent stem cells, comprising culturing pluripotent stem cells in conditions maintaining their state of being unspecialized and differentiation- pluripotent, characterized in that by the use of a liquid culture medium and a culture vessel having a given concentration of molecules capable of adhering to the pluripotent stem cells applied to a substrate solid phase surface through immobilization or coating, proliferation or gene introduction/expression for the pluripotent stem cells is carried out in dispersed form while maintaining their state of being unspecialized and differentiation-pluripotent without the use of any feeder cells.
Il est prévu un procédé de prolifération de cellules souches pluripotentes, comme les cellules ES. Il est prévu des procédés de prolifération et d'introduction de gènes pour cellules souches pluripotentes, consistant en la mise en culture de cellules souches pluripotentes dans des conditions maintenant leur état non spécialisé et de pluripotence en différentiation, caractérisés en ce que l'utilisation d'un milieu de culture liquide et un récipient de culture d'une concentration donnée de molécules capables d'adhérer aux cellules souches pluripotentes appliquées à une surface de substrat en phase solide par immobilisation ou revêtement, la prolifération ou l'introduction/l'expression de gènes pour les cellules souches pluripotentes s'effectue sous forme dispersée tout en préservant leur état non spécialisé et de pluripotence en différentiation sans utiliser de cellules d'alimentation.
Akaike Toshihiro
Fukuda Keiichi
Koshimizu Uichi
Nagaoka Masato
Akaike Toshihiro
Daiichi Asubio Pharma Co. Ltd.
Daiichi Sankyo Company Limited
Fukuda Keiichi
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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