Pna chip using plastic substrate coated with epoxy...

Details Pna chip using plastic substrate coated with epoxy... Pna chip using plastic substrate coated with epoxy...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2563948

Provided is a PNA (Peptide Nucleic Acid) chip in which a probe PNA containing a desired DNA sequence is immobilized on a plastic substrate coated with an epoxy group-containing polymer. Therefore, single-stranded PNAs can be immobilized on a transparent plastic substrate by means of an epoxy group- containing polymer layer in an efficient and cost-effective manner. Fluorescence signal detection based on PNA/DNA hybridization enables identification of SNP (Single Nucleotide Polymorphism).

L'invention concerne une puce à PNA (acide nucléique peptidique) dans laquelle une sonde PNA contenant une séquence d'ADN désirée est immobilisée sur un substrat en plastique revêtu d'un polymère contenant un groupe époxyde. Ainsi, les PNA monocaténaires peuvent être immobilisés de manière efficace et rentable sur un substrat en plastique transparent, au moyen d'une couche de polymère contenant un groupe époxyde. Une détection de signal par fluorescence basée sur une hybridation PNA/ADN permet d'identifier un SNP (polymorphisme mononucléotidique).

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