Poliovirus replicons encoding therapeutic agents and uses...

A - Human Necessities – 61 – K

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A61K 48/00 (2006.01) A61K 38/19 (2006.01) A61P 25/00 (2006.01) A61P 31/04 (2006.01) A61P 35/00 (2006.01) C12N 15/86 (2006.01)

Patent

CA 2409190

The invention pertains to methods of delivering a polypeptide to a cell comprising: (a) contacting a cell with a replicon having a non-poliovirus nucleic acid substituted for a nucleic acid which encodes at least a portion of a protein necessary for encapsidation, the non-poliovirus nucleic acid encoding, in an expressible form, a polypeptide or fragment thereof; and (b) maintaining the cells under conditions appropriate for introduction of the replicons into the cells. The cell may be within a subject and the polypeptide may be a therapeutic agent. The methods of the invention may be used to treat diseases including central nervous system disorders, infectious diseases, and cancer.

Cette invention se rapporte à des procédés d'apport d'un polypeptide dans une cellules, qui consiste: (a) à mettre en contact une cellule avec un réplicon contenant un acide nucléique non poliovirus remplaçant un acide nucléique, qui code au moins une partie d'une protéine nécessaire à l'encapsidation, l'acide nucléique non poliovirus codant, sous une forme expressible, un polypeptide ou un fragment de celui-ci; et (b) à maintenir ces cellules dans des conditions propres à introduire les réplicons dans lesdites cellules. La cellule peut faire partie de l'organisme d'un sujet et le polypeptide peut être un agent thérapeutique. Ces procédés peuvent servir à traiter des maladies telles que les troubles du système nerveux central, les maladies infectieuses et le cancer.

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