Polistinae venom phospholipase

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/18 (2006.01) A61K 39/35 (2006.01) C12N 9/20 (2006.01) C12N 9/26 (2006.01) C12N 15/10 (2006.01) C12N 15/54 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2344578

A unique clone of a Polistinae venom enzyme, recombinantly produced Polistinae venom enzymes, and methods of using the recombinant enzymes are provided. In a specific example, both phospholipase and hyaluronidase cDNA from Polistes annulares contain apparent "intronic" sequences. In still a further embodiment, genetic engineering permits the construction of the "intronic" sequences to yield a useful coding sequence for expression of mature Polistinae venom enzyme proteins.

On décrit un clone unique d'une enzyme de venin de Polistinae, des enzymes de venin de Polistinae produites par recombinaison, et des procédés d'utilisation des enzymes de recombinaison. Dans un exemple spécifique, des ADNc phospholipase et hyaluronidase provenant de Polistes annulares contiennent tous les deux des séquences "introniques" apparentes. Dans une autre forme de réalisation, on peut produire, par génie génétique, les séquences "introniques" pour produire une séquence de codage utile servant à l'expression de protéines d'enzyme de venin de Polistinae à l'état mature.

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