Pollen specific promoter

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01) C12N 5/04 (2006.01) C12N 5/10 (2006.01) C12N 15/29 (2006.01) C12N 15/55 (2006.01)

Patent

CA 2319079

A recombinant nucleic acid which comprises a promoter sequence of the ZmC5 gene in maize, or a variant or fragment thereof, which acts as a promoter in pollen. The DNA sequence of the promoter is depicted in figure 5. Expression cassettes and expression systems as well as transformation methods, transformed plants including the promoter sequence of the invention are also claimed. The nucleic acid may be used inter alia in the production of male sterile plants and/or hybrids as well as in the transformation of pollen.

L'invention concerne un acide nucléique recombiné, comprenant une séquence promoteur du gène ZmC5 du maïs, ou une variante ou un fragment de celui-ci, agissant comme promoteur dans le pollen, la séquence d'ADN de ce promoteur étant illustrée dans la figure 5. L'invention concerne également des cassettes et des systèmes d'expression, ainsi que des méthodes de transformation et des végétaux transformés comprenant la séquence promoteur susmentionnée. L'acide nucléique peut être utilisé inter alia pour produire des végétaux et/ou des hybrides à stérilité mâle, et pour transformer le pollen.

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