G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/543 (2006.01)
Patent
CA 2580922
The present invention is directed to a pre-coated substrate, such as a slide, that is useful for immobilizing a sample. The invention is further provides methods of preparing such pre-coated substrates and methods of analyzing biological samples immobilized on such pre-coated substrate. The substrate is coated with a polycationic polymeric coating material specifically selected such that that coated substrate exhibits increased stability and prolonged shelf-life. Preferred polymeric coating materials include allylic or vinylic polymers having cationic groups thereon and having no more than a small percentage of peptidic monomeric linkages, particularly polydiallyldimethylammonium (PDDA).
La présente invention concerne un substrat pré-revêtu, tel qu'une plaquette, utile pour immobiliser un échantillon. L'invention concerne également des procédés destinés à préparer des substrats pré-revêtus et des procédés d'analyse d'échantillons biologiques immobilisés sur ces substrats pré-revêtus. Le substrat est revêtu d'une matière de revêtement polymère polycationique sélectionnée spécifiquement de sorte que le substrat revêtu présente une stabilité accrue et une durée de vie prolongée. Les matières de revêtements polymères préférées incluent des polymères allyliques ou vinyliques comportant des groupes cationiques et présentant un faible pourcentage de liaisons monomériques peptidiques, et notamment un polydiallyldiméthylammonium (PPDA).
Fox William Alan
Ray William Carl III
Smart & Biggar
Tripath Imaging Inc.
LandOfFree
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