Polyethylene glycol in double antibody radioimmunoassay

Details Polyethylene glycol in double antibody radioimmunoassay Polyethylene glycol in double antibody radioimmunoassay

G - Physics

01

N

167/41

G01N 33/539 (2006.01) G01N 33/53 (2006.01)

Patent

CA 1145672

877-170F GG:280 DOUBLE ANTIBODY SEPARATION METHOD ABSTRACT A double antibody radioimmunoassay method for determining antigens in a sample wherein polyethylene glycol is provided in a reaction medium to accelerate an immunoprecipitation reaction between water-soluble first antibodies bound to antigen and water-soluble second antibodies which are not specific to said antigens but which are specific to said first antibodies. A double antibody, multi-component radioimmunoassay test kit is also provided. The kit contains radiolabeled antigen reagent, first water-soluble antibody reagent and second water- soluble antibody reagent, as well as a polyethylene glycol reagent, which may optionally be combined with the second antibody reagent, in an amount sufficient to accelerate an immunoprecipitation reaction, between said first and second reagents.

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