Polyhdroxyalkanoate biopolymer compositions

C - Chemistry – Metallurgy – 12 – P

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C12P 7/62 (2006.01) C12N 15/00 (2006.01) C12N 9/02 (2006.01) C12N 9/10 (2006.01) C12N 15/63 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2726139

Several novel PHA polymer compositions produced using biological sytems include monomers such as 3-hydroxybutyrate, 3-hydroxypropionate, 2-hydroxybutyrate, 3-hydroxyvalerate, 4- hydroxybutyrate, 4-hydroxyvalerate and 5-hydroxyvalerate. These PHA compositions can readily be extended to incorporate additional monomers including, for example, 3-hydroxyhexanoate, 4-hydroxyhexanoate, 6-hydroxyhexanoate or other longer chain 3--hydroxyacids containing seven or more carbons. This can be accomplished by taking natural PHA producers and mutating through chemical or transposon mutagenesis to delete or inactivate genes encoding undesirable activities. Alternatively, the strains can be genetically engineered to express only those enzymes required for the production of the desired polymer composition.; Methods for genetically engineering PHA producing microbes are widely known in the art (Huisman and Madison, 1998, Microbiology and Molecular Biology Reviews, 63: 21-53). These polymers have a variety of uses in medical, industrial and other commercial areas.

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