Polyhydroxyalkanoate biopolymer compositions

C - Chemistry – Metallurgy – 12 – P

Patent

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C12P 7/62 (2006.01) C08G 63/06 (2006.01) C12N 1/21 (2006.01) C12N 9/00 (2006.01) C12N 9/10 (2006.01) C12N 15/00 (2006.01) C12N 15/63 (2006.01) C12N 15/70 (2006.01)

Patent

CA 2328471

Several novel PHA polymer compositions produced using biological sytems include monomers such as 3-hydroxybutyrate, 3-hydroxypropionate, 2- hydroxybutyrate, 3-hydroxyvalerate, 4-hydroxybutyrate, 4-hydroxyvalerate and 5- hydroxyvalerate. These PHA compositions can readily be extended to incorporate additional monomers including, for example, 3-hydroxyhexanoate, 4- hydroxyhexanoate, 6-hydroxyhexanoate or other longer chain 3-hydroxyacids containing seven or more carbons. This can be accomplished by taking natural PHA producers and mutating through chemical or transposon mutagenesis to delete or inactivate genes encoding undesirable activities. Alternatively, the strains can be genetically engineered to express only those enzymes required for the production of the desired polymer composition. Methods for genetically engineering PHA producing microbes are widely known in the art (Huisman and Madison, 1998, Microbiology and Molecular Biology Reviews, <u>63</u>: 21-53). These polymers have a variety of uses in medical, industrial and other commercial areas.

L'invention concerne plusieurs nouvelles compositions polymères de PHA (polyhydroxyalcynoate) obtenues grâce à des systèmes biologiques et comprenant des monomères tels que 3-hydroxybutyrate, 3-hydroxypropionate, 2-hydroxybutyrate, 3-hydroxyvalérate, 4-hydroxybutyrate, 4-hydroxyvalérate et 5-hydroxyvalérate. On peut facilement étendre ces compositions de PHA de sorte qu'elles comprennent des monomères supplémentaires comme par exemple 3-hydroxyhexanoate, 4-hydroxyhexanoate, 6-hydroxyhexanoate ou une autre chaîne plus longue de 3-hydroxyacides contenant sept ou plusieurs carbones. On peut réaliser cette opération en prenant des producteurs de PHA naturels et en effectuant une mutation par mutagenèse chimique ou par transposon pour effacer ou inactiver des gènes codant des activités non voulues. Dans un autre mode de réalisation, on peut mettre au point les souches par génie génétique de sorte qu'elles n'expriment que les enzymes nécessaires à la production de la composition polymère voulue. Des méthodes de mise au point par génie génétique de microbes produisant PHA sont bien connues dans le milieu (Huisman et Madison, 1998, Microbiology and Molecular Biology Reviews, <u>63</u>: 21-53). Ces polymères peuvent avoir diverses applications dans les domaines médical, industriel et dans d'autres domaines commerciaux.

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