C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 7/42 (2006.01) C12N 15/00 (2006.01) C12N 15/52 (2006.01) C12P 7/62 (2006.01)
Patent
CA 2339351
Organisms are provided which express enzymes such as glycerol dehydratase, diol dehydratase, acyl-CoA transferase, acyl-CoA synthetase .beta.-ketothiolase, acetoacetyl-CoA reductase, PHA synthase, glycerol-3-phosphate dehydrogenase and glycerol-3-phosphatase, which are useful for the production of PHAs. In some cases one or more of these genes are native to the host organism and the remainder are provided from transgenes. These organisms produce poly(3- hydroxyalkanoate) homopolymers or co-polymers incorporating 3-hydroxypropionate or 3-hydroxyvalerate monomers wherein the 3- hydroxypropionate and 3-hydroxyvalreate units are derived from the enzyme catalysed conversion of diols. Suitable diols that can be used include 1,2-propanediol, 1,3-propanediol and glycerol. Biochemical pathways for obtaining the glycerol from normal cellular metabolites are also described. The PHA polymers are readily recovered and industrially useful as polymers or as starting materials for a range of chemical intermediates including 1,3-propanediol, 3-hydroxypropionaldehyde, acrylics, malonic acid, esters and amines.
L'invention concerne des organismes exprimant des enzymes telles que glycérol déshydratase, diol déshydratase, acyl-CoA transférase, acyl-CoA synthétase beta -cétothiolase, acétoacétyl-CoA réductase, PHA synthase, glycérol-3-phosphate déshydrogénase et glycérol-3-phosphatase, qui s'utilisent pour la production de PHA (polyhydroxyalcanoates). Dans certains cas, un ou plusieurs de ces gènes sont endogènes aux organismes hôtes et les autres proviennent de transgènes. Ces organismes produisent des homopolymères ou copolymères poly(3-hydroxyalcanoate) comprenant des monomères 3-hydroxypropionate ou 3-hydroxyvalérate, les unités 3-hydroxypropionate et 3-hydroxyvalérate étant dérivées d'une conversion de diols par catalysation d'enzymes. On peut utiliser de manière appropriée des diols tels que 1,2-propanediol, 1,3-propanediol et glycérol. L'invention concerne également des voies biochimiques de production du glycérol à partir de métabolites cellulaires normaux. Les polymères de PHA sont facilement récupérés et s'utilisent dans l'industrie comme polymères ou comme matières premières dans une gamme d'intermédiaires chimiques tels que 1,3-propanediol, 3-hydroxypropionaldéhyde, des acryliques, de l'acide malonique, des esters et des amines.
Peoples Oliver P.
Skraly Frank
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Metabolix Inc.
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