Polyketides and their synthesis

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/52 (2006.01) C07D 311/00 (2006.01) C07D 321/00 (2006.01) C07D 493/08 (2006.01) C12N 1/21 (2006.01) C12N 15/76 (2006.01) C12P 19/62 (2006.01)

Patent

CA 2318365

The present invention provides an extended polyketide synthase gene assembly comprising a loading module and a plurality of extension modules wherein a second nucleic acid portion, encoding at least one extension module, has been inserted into a first nucleic acid portion encoding the loading module and at least one, and preferably a plurality, of extension modules. Such extended genes are useful in the production of novel macrolides of larger size than the natural product, for example 16-membered ring macrolides instead of 14- membered ring macrolides.

La présente invention concerne un groupe de gènes polycétide-synthase allongés, qui comprend un module de charge et une pluralité de modules d'élongation dans lesquels une deuxième partie d'acide nucléique, codant au moins un module d'élongation, a été insérée dans une première partie d'acide nucléique codant le module de charge et au moins un, ou de préférence plusieurs, module d'élongation. Lesdits gènes allongés conviennent pour la production de nouveaux macrolides présentant une taille plus grande que celle du produit naturel, par exemple des macrolides présentant un cycle à 16 chaînons au lieu de macrolides présentant un cycle à 14 chaînons.

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