C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/52 (2006.01) C07K 19/00 (2006.01) C12N 9/02 (2006.01) C12N 9/04 (2006.01) C12N 9/10 (2006.01) C12N 9/88 (2006.01) C12N 15/62 (2006.01) C12P 17/06 (2006.01) C12P 19/62 (2006.01)
Patent
CA 2332491
Nucleic acid molecules encoding at least part of a Type I polyketide synthase, and having a polylinker with multiple restriction enzyme sites in place of one or more PKS genes encoding enzymes associated with reduction, optionally further including nucleic acid incorporated into the polylinker, the further nucleic acid encoding one or more reductive enzymes; plasmids incorporating such nucleic acids; host cells transfected with such plasmids; methods relating thereto.
L'invention concerne des molécules d'un acide nucléique codant au moins partiellement pour une polycétide-synthase du type I et présentant un lieur à multiples sites d'enzyme de restriction au lieu d'un ou plusieurs gènes PKS codant pour des enzymes associés avec la réduction. Un autre acide nucléique codant pour une ou plusieurs enzymes de réduction peut être éventuellement introduit dans le lieur multisite. L'invention concerne également des plasmides contenant lesdits acides nucléiques, des cellules hôtes transfectées au moyen desdits plasmides ainsi que des procédés associés.
Kellenberger Johannes Laurenz
Leadlay Peter Francis
Mcarthur Hamish Alastair Irvine
Staunton James
Stutzman-Engwall Kim Jonelle
Biotica Technology Limited
Fetherstonhaugh & Co.
Pfizer Inc.
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