Polymerase cleavage agents and detection of rna

Details Polymerase cleavage agents and detection of rna Polymerase cleavage agents and detection of rna

C - Chemistry, Metallurgy

12

N

C12N 9/22 (2006.01) C12N 9/12 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2410072

The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5' nuclease activity of a variety of enzymes is used to cleave a target- dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

La présente invention se rapporte à de nouveaux agents de clivage et polymérases destinés au clivage et à la modification d'un acide nucléique. Ces agents de clivage et polymérases s'avèrent utiles, notamment, pour la détection et la caractérisation de séquences d'acides nucléiques et de variations dans des séquences d'acides nucléiques. Dans certaines réalisations, l'activité de nucléase 5' d'une variété d'enzymes est utilisée pour couper une structure à clivage dépendant de la cible, ce qui permet d'indiquer la présence de séquences d'acides nucléiques spécifiques ou de variations spécifiques de ces séquences.

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