C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 19/34 (2006.01) C12N 9/12 (2006.01) C12N 9/16 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2283635
The invention provides novel extracts, proteins, and complexes that improve the polymerization activity of nucleic acid polymerases. Included within the aspects of the invention are methods for identifying compositions with a polymerase enhancing activity, methods for purifying and using these compositions, and specific extracts, proteins, and complexes that function to enhance polymerase activity. As an example, specifically described is nucleotide and amino acid sequence information for a Pyrococcus furiousus PEF (P45), which was used to produce a recombinant PEF.
L'invention concerne des nouveaux extraits, protéines et complexes améliorant l'activité de polymérisation de polymérases d'acides nucléiques. L'invention comporte plusieurs aspects, en particulier des procédés d'identification de compositions possédant une activité de stimulation de la polymérase, des procédés de purification et d'utilisation de ces compositions, et des extraits, des protéines et des complexes spécifiques, qui ont pour fonction de stimuler l'activité de la polymérase. Sont décrites spécifiquement, à titre d'exemple, les informations de séquences d'acides aminés et de nucléotides d'une PEF de Pyrococcus furiousus (P45), que l'on a utilisées pour produire une PEF de recombinaison.
Hansen Connie J.
Hogrefe Holly
Kirby Eades Gale Baker
Stratagene
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