Polymerase inhibitor and method of using same

Details Polymerase inhibitor and method of using same Polymerase inhibitor and method of using same

C - Chemistry, Metallurgy

07

H

C07H 21/04 (2006.01) C12N 15/11 (2006.01)

Patent

CA 2521127

The present invention provides nucleic acid based polymerase inhibitors and methods for reducing non-specific polymerase extension and amplification in nucleic acid amplification reactions. The polymerase inhibitors provide a double stranded nucleic acid portion that is recognized by a polymerase enzyme as a template for extension but is incapable of being extended by the polymerase enzyme. The polymerase binds to the polymerase inhibitor which sequesters the enzyme until the temperature achieves a level that denatures the double stranded portion of the inhibitor after which the polymerase is released and can then catalyze nucleic acid extension.

L'invention concerne des inhibiteurs de polymérase à base d'acide nucléique, ainsi que des procédés de réduction de l'extension de polymérase non spécifique et de l'amplification dans des réactions d'amplification d'acide nucléique. Les inhibiteurs de polymérase fournissent une portion d'acide nucléique bicaténaire qui est reconnue par une enzyme polymérase, comme matrice pour l'extension, mais incapable d'extension par l'enzyme polymérase. La polymérase se lie à l'inhibiteur de polymérase qui séquestre l'enzyme jusqu'à ce que la température atteigne un niveau qui dénature la portion bicaténaire de l'inhibiteur, après quoi la polymérase est libérée et peut alors catalyser l'extension de l'acide nucléique.

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