Polymerase signaling assay

C - Chemistry – Metallurgy – 12 – Q

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C12Q 1/68 (2006.01)

Patent

CA 2331788

The present invention relates to the field of nucleic acid sequence analysis. The invention provides methods for performing a polymerase signal assay (PSA) to analyze nucleotide sequences using solid phase sequence arrays comprising a plurality of sequence reagents with primer sequences 4-6 bases in length. The methods of the invention generate a binary signal pattern which can be used to identify nucleic acid sequences and/or mutations and polymorphisms of a nucleic acid sequence. Mutations and polymorphisms which can be identified by the methods of the invention include single nucleotide polymorphisms (SNP's), base deletions, base insertions, and heterozygous as well homozygous polymorphisms.

La présente invention concerne le domaine de l'analyse de séquences d'acide nucléique. L'invention concerne des méthodes permettant d'exécuter un dosage de signal de polymérase (PSA) afin d'analyser des séquences nucléotidiques à l'aide d'ensembles de séquences en phase solide comprenant une pluralité de réactifs de séquences avec une longueur de 4 à 6 bases de séquences d'amorce. Les méthodes de l'invention produisent un type de signal binaire pouvant être utilisé pour identifier des séquences d'acide nucléique et/ou des mutations ainsi que des polymorphismes d'une séquence d'acide nucléique. Les mutations et les polymorphismes que les méthodes de l'invention peuvent identifier comprennent les polymorphismes mononucléotidiques (SNP), les délétions de base, les insertions de base ainsi que les polymorphismes hétérozygotes et homozygotes.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Polymerase signaling assay does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Polymerase signaling assay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Polymerase signaling assay will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-1591810

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.