Polymerase stabilization

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/12 (2006.01) C12N 9/96 (2006.01)

Patent

CA 2689068

The present invention relates to methods and compositions for providing purified thermostable enzymes, particularly thermostable DNA polymerases, that are free of exogenous detergents The present invention also provides methods for providing such purified thermostable DNA polymerases to assays in an active form by adding one or more detergents The present invention further provides compositions and kits comprising purified thermostable DNA polymerases for use in a variety of applications, including amplification and sequencing of nucleic acids.

La présente invention concerne des procédés et des compositions permettant de fournir des enzymes thermostables purifiées, et plus précisément des ADN polymérases thermostables, exemptes de détergents exogènes. L'invention a également trait à des procédés permettant d'introduire lesdits ADN polymérases dans des dosages de manière active, par l'ajout d'un ou de plusieurs détergents. En outre, l'invention se rapporte à des compositions et des kits comprenant des ADN polymérases thermostables purifiés à utiliser dans une variété d'applications, notamment dans l'amplification et le séquençage d'acides nucléiques.

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