Polymeric arrays and methods for their use in binding assays

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) B01J 19/00 (2006.01) C07H 21/00 (2006.01) C07H 21/04 (2006.01) C07B 61/00 (2006.01)

Patent

CA 2312848

Arrays of polymeric targets stably associated with the surface of a rigid solid support are provided. In the subject arrays, the polymeric targets are arranged at least according to size. The polymeric targets of the subject arrays are generally biopolymeric compounds, e.g. nucleic acids and proteins, where ribonucleic acids and proteins are the preferred polymeric targets. The subject arrays find use in a variety of different applications, and are particularly suited for use in high through gene expression analysis applications.

L'invention concerne des réseaux de cibles polymères associées de façon stable à la surface d'un support solide rigide. Dans les réseaux en question, les cibles polymères sont aménagées au moins en fonction de leur taille. Les cibles polymères des réseaux en question sont généralement des composés biopolymères, p. ex. des acides nucléiques et des protéines, les acides ribonucléiques et les protéines constituant des cibles polymères préférées. Les réseaux en question sont utiles dans diverses applications différentes, et conviennent particulièrement pour des applications d'analyse d'expression génique de haut rendement.

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