Polymeric fluorophores enhanced by moieties providing a...

C - Chemistry – Metallurgy – 09 – B

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C09B 69/10 (2006.01) C09B 23/10 (2006.01) C09B 23/14 (2006.01) C12Q 1/04 (2006.01) G01N 15/14 (2006.01) G01N 21/64 (2006.01) G01N 33/533 (2006.01)

Patent

CA 2244768

Fluorescent conjugates suitable for use in flow cytometry and other biological applications. The fluorescent conjugates comprise an antibody having a polymeric dye bound thereto. The polymeric dye is preferably enhanced by a hydrophobic and conformationally restrictive moiety either bound thereto or in close association therewith. The hydrophobic and conformationally restrictive moiety is preferably derived from a cyclodextrin. The polymeric dye comprises a polymeric entity having signal-generating groups, such as aminostyryl pyridinium dye residues attached thereto. The fluorescent conjugates exhibit exceptional stability characteristics and avoid many of the problems of energy transfer, bio-conjugability, and solubility.

Cette invention se rapporte à des conjugués fluorescents se prêtant à une utilisation en cytométrie en flux et dans d'autres applications biologiques. Ces conjugués fluorescents comprennent un anticorps auquel est lié un colorant polymère. Ce colorant polymère est de préférence amélioré par une fraction hydrophobe et à restriction conformationnelle qui est soit liée au colorant soit étroitement associée à lui. Cette fraction hydrophobe et à restriction conformationnelle est de préférence dérivée d'une cyclodextrine. Ledit colorant polymère contient une entité polymère à laquelle sont fixés des groupes générateurs de signaux, tels que des restes de colorant aminostyrylpyridinium. Ces conjugués fluorescents possèdent des caractéristiques de stabilité exceptionnelles et permettent d'éviter les nombreux problèmes de transfert d'énergie, de bioconjugabilité et de solubilité.

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