Polymeric sorbent sheets as ion reservoirs for electroblotting

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 1/26 (2006.01) C07H 21/00 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01) G01N 27/447 (2006.01)

Patent

CA 2709213

Electroblotting for the transfer of electrophoretically separated species from a gel to a transfer membrane is performed in a semi-dry format with sheets of absorbent polyester, polyester/cellulose blend, or polyurethane-coated cellulose wetted with buffer solution in place of the traditional buffer-wetted filter paper. The result is effective electroblotting at a lower electric current level than that obtained with filter paper and thereby less resistance heating of the gel, the transfer membrane, and the species being transferred.

Selon l'invention, l'électrobuvardage permettant le transfert d'espèces séparées par électrophorèse d'un gel à une membrane de transfert est réalisé dans un mode semi-humide au moyen de feuilles de polyester absorbant, d'un mélange polyester/cellulose ou de cellulose revêtue de polyuréthanne humidifiées par une solution tampon à la place du papier filtre humidifié par tampon traditionnel. Il en résulte un électrobuvardage efficace à un niveau de courant électrique inférieur à celui obtenu avec le papier filtre, ce qui réduit le chauffage par résistance du gel, de la membrane de transfert et des espèces transférées.

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