Polymorphisms in the human cyp2d6 gene promoter region and...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/63 (2006.01) C07K 14/47 (2006.01) C12N 9/02 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2398104

Provided are polynucleotides of molecular variant promoters of the CYP2D6 gene which, for example, are associated with abnormal drug response or individual predisposition to several common diseases and disorders caused by drug under- or over-metabolization, and vectors comprising such polynucleotides. Furthermore, methods of diagnosing the status of disorders related to intermediate metabolization of drugs are described. In addition, kits comprising oligonucleotides hybridizing to the CYP2D6 promoter and/or being capable of being extended into this region useful for diagnosing subjects that are ultrarapid or intermediate metabolizer of drugs are provided.

L'invention concerne des polynucléotides des promoteurs de variant moléculaire du gène CYP2D6, associés, entre autres, à une réaction anormale aux médicaments ou à une prédisposition individuelle à plusieurs maladies et troubles courants provoqués par une métabolisation de médicaments insuffisante ou excessive, ainsi que des vecteurs comprenant ces polynucléotides. L'invention concerne également des techniques de diagnostic de l'état des troubles associés à la métabolisation intermédiaire de médicaments. L'invention concerne en outre des kits comprenant des oligonucléotides s'hybridant au promoteur de CYP2D6 et/ou pouvant s'étendre dans cette région, utiles dans le diagnostic de sujets présentant une métabolisation ultra-rapide ou intermédiaire de médicaments.

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