Polynomial amplification of nucleic acids

Details Polynomial amplification of nucleic acids Polynomial amplification of nucleic acids

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2476564

The invention provides methods and compositions for the amplication and replication of nucleic acid molecules. In particular, novel amplification methods, referred to herein as polynomial amplification, are provided. According to these methods, a nucleic acid molecule to be amplified is contacted with at least two primer; a non-replicable primer which may hybridize to the nucleic acid molecule being amplified, and a replicable primer which may hybridize to a primer extension product generated from extension of the non-replicable primer.

L'invention concerne des méthodes et des compositions destinées à l'amplification et à la réplication de molécules d'acides nucléiques. Notamment, ladite invention a trait à de nouvelles méthodes d'amplifications, auxquelles on se réfère ici en tant qu'amplification polynomiale. Selon ces méthodes, on met en contact une molécule d'acide nucléique à amplifier avec au moins deux amorces, une amorce non réplicable pouvant s'hybrider à la molécule d'acide nucléique en cours d'amplification et une amorce réplicable pouvant s'hybrider à un produit d'extension d'amorce généré à partir de l'extension de l'amorce non réplicable.

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