Polynucleotide aptamer-based cross-linked materials and uses...

A - Human Necessities – 61 – K

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Details

A61K 47/48 (2006.01) C12N 15/115 (2010.01) A61K 31/7088 (2006.01) A61P 3/08 (2006.01) C07H 21/00 (2006.01)

Patent

CA 2750245

In one aspect, the disclosure provides cross-linked materials that include multivalent polynucleotide aptamers that bind a target molecule; and conjugates that include two or more separate affinity ligands bound to a conjugate framework, wherein the two or more affinity ligands compete with the target molecule for binding with the aptamers and wherein conjugates are cross-linked within the material as a result of non-covalent interactions between aptamers and affinity ligands on different conjugates. These materials are designed to release amounts of conjugate in response to desired concentrations of the target molecule. De-pending on the end application, in various embodiments, the conjugates may also include a drug and/or a detectable label. The drug, detectable label and affinity ligands may be covalently or non- covalently bound to the conjugate framework.

L'invention concerne des matières réticulées qui comprennent des aptamères polynucléotidiques multivalents qui se lient à une molécule cible, et des conjugués qui comprennent au moins deux ligands d'affinité séparés liés à un squelette de conjugué, au moins deux des ligands d'affinité concurrençant la molécule cible pour se lier aux aptamères, et les conjugués étant réticulés à l'intérieur de la matière suite à des interactions non covalentes entre des aptamères et des ligands d'affinité sur différents conjugués. Ces matières sont conçues pour libérer des doses de conjugués par rapport à des concentrations désirées de la molécule cible. En fonction de l'application finale, selon différents modes de mise en oeuvre, les conjugués peuvent également comprendre un médicament et/ou une étiquette détectable. Le médicament, l'étiquette détectable et les ligands d'affinité peuvent être liés de manière covalente ou non covalente au squelette du conjugué.

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