Polynucleotide arrays and their use in sequencing

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2398168

A device for use in polynucleotide sequencing procedures comprises an array of polynucleotide molecules capable of interrogation and immobilised on a solid surface, wherein the array has a density which allows the molecules to be individually resolved. The molecules on the array comprise a target polynucleotide and a primer sequence, the primer being maintained in spatial relationship to the target, both being linked via a covalent bond. The device may be used in genotyping experiments, in which an array of genomic DNA is prepared to allow multiple sequencing reactions to be performed.

L'invention concerne un dispositif destiné à être utilisé dans des procédés de séquençage de polynucléotides, comprenant un groupe de molécules polynucléotidiques pouvant être interrogé ou immobilisé sur une surface solide, le groupe ayant une densité permettant aux molécules d'être individuellement résolues. Les molécules du groupe comprennent un polynucléotide cible et une séquence d'amorce, l'amorce étant maintenue en relation spatiale par rapport à la cible, tous deux étant liés par l'intermédiaire d'une liaison covalente. Ce dispositif peut être utilisé dans des expériences visant à déterminer le génotype, dans lesquelles un groupe d'ADN génomique est préparé afin que des réactions de séquençage multiples puissent être effectuées.

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