Polynucleotide capture materials, and methods of using same

Details Polynucleotide capture materials, and methods of using same Polynucleotide capture materials, and methods of using same

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12Q 1/68 (2006.01) G01N 1/40 (2006.01)

Patent

CA 2693654

Methods for processing polynucleotide-containing biological samples, and materials for capturing polynucleotide molecules such as RNA and/or DNA from such samples. The RNA and/or DNA is captured by polyamidoamine (PAMAM(Gener-ation 0)) bound to a surface, such as the surface of magnetic particles. The methods and materials have high efficiency of binding RNA and of DNA, and of release, and thereby permit quantitative determinations.

L'invention concerne des procédés pour le traitement d'échantillons biologiques contenant des polynucléotides, et des matériaux pour absorber les molécules de polynucléotides telles que l'ARN et/ou l'ADN provenant de tels échantillons. L'ARN et/ou l'ADN sont absorbés par la poly(amidoamine) (PAMAM (génération 0)) liée à une surface, telle que la surface de particules magnétiques. Les procédés et matériaux ont une efficacité élevée de liaison d'ARN et d'ADN, et de libération, et permettent ainsi des déterminations quantitatives.

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