Polynucleotide compositions

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/88 (2006.01) A61K 9/107 (2006.01) A61K 31/70 (2006.01) A61K 31/7088 (2006.01) A61K 47/34 (2006.01) A61K 47/48 (2006.01) A61K 48/00 (2006.01) C08G 65/00 (2006.01) C08G 81/00 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2205486

The invention provides compositions for stabilizing polynucleic acids and increasing the ability of polynucleic acids to cross cell membranes and act in the interior of a cell. In one aspect, the invention provides a polynucleotide complex between a polynucleotide and certain polyether block copolymers. Preferably the polynucleotide complex will further include a polycationic polymer. In another aspect, the invention provides a polynucleotide complex between a polynucleotide and a block copolymer comprising a polyether block and a polycation block. In yet another aspect, the invention provides polynucleotides that have been covalently modified at their 5' or 3' end to attach a polyether polymer segment. In still another aspect, the invention provides certain preferred polycationic polymers.

Composés visant à stabiliser les acides polynucléiques et à accroître leur capacité à traverser les membranes des cellules pour agir à l'intérieur de celles-ci. Dans un premier temps, un complexe polynucléotidique est constitué à partir d'un polynucléotide et de copolymères séquencés de polyéther données; il est souhaitable que le complexe polynucléotidique contienne un polymère polycationique. Un complexe nucléotidique peut ensuite être constitué à partir d'un polynucléotide et d'un copolymère séquencé contenant un polyéther séquencé et un polycation séquencé. Un autre composé est constitué de polynucléotides dont les extrémités 5' ou 3' ont été modifiées de manière covalente, de façon à pouvoir y raccorder un segment polymère polyéther. Enfin, s'ajoutent à ces composés certains polymères cationiques qu'il est préférable d'employer.

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