Polynucleotide constructs and uses thereof

Details Polynucleotide constructs and uses thereof Polynucleotide constructs and uses thereof

C - Chemistry, Metallurgy

12

N

C12N 15/63 (2006.01) A61K 48/00 (2006.01) C07K 14/47 (2006.01) C12N 5/10 (2006.01) C12N 7/01 (2006.01) C12N 15/86 (2006.01)

Patent

CA 2343324

A polynucleotide is provided which comprises at least two repeats of a hypoxia response element (HRE), wherein the hypoxia-inducible factor consensus binding sites within each of the two repeats are separated by a spacer of at least 20 contiguous nucleotides. A polynucleotide is also provides which comprised at least three repeats of a phosphoglycerate kinase (PGK) hypoxia response element (HRE) operably linked to an SV40 promoter or an MLV promoter. The polynucleotide may be operably linked to a nucleic acid of interest to drive expression of the NOI in a cell under hypoxic conditions.

L'invention concerne un polynucléotide comprenant au moins deux répétitions d'un élément de réponse hypoxique (HRE), les sites de liaison consensus au facteur inductible par hypoxie dans chacune de ces deux répétitions étant séparés par un espaceur d'au moins 20 nucléotides contigus. Cette invention concerne également un polynucléotide comprenant au moins trois répétitions d'un élément de réponse hypoxique (HRE) de phosphoglycérokinase (PGK), lié de manière fonctionnelle à un promoteur SV40 ou à un promoteur MLV. Ce polynucléotide peut être lié de manière fonctionnelle à un acide nucléique recherché afin de diriger l'expression de l'acide nucléique recherché (NOI) dans une cellule, dans des conditions hypoxiques.

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