Polynucleotide constructs for increased lysine production

Details Polynucleotide constructs for increased lysine production Polynucleotide constructs for increased lysine production

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) C12N 1/20 (2006.01) C12N 9/02 (2006.01) C12N 9/12 (2006.01) C12N 15/52 (2006.01) C12N 15/53 (2006.01) C12N 15/63 (2006.01) C12P 13/08 (2006.01)

Patent

CA 2437656

The invention relates to production of lysine, and provides several isolated polynucleotide molecules useful for the production of L-lysine. One such polynucleotide encodes an aspartate kinase (ask), an aspartate-semialdehyde dehydrogenase (asd) and a dihydrodipicolinate reductase (dapB). Other polypeptides encode ask, asd, dapB and a diaminopimelate dehydrogenase (ddh); ask, asd, dapB, ddh and an ORF2 poypeptide; and ask, asd dapB, ddh, ORF2 and a diaminopimelate decarboxylase (lysA). The invention further provides methods of making and using the polynucleotides, and methods to increase the production of L-lysine.

L'invention concerne la production de lysine et plusieurs molécules de polynucléotides isolées utilisées dans la production de L-lysine. Un tel polynucléotide code une aspartate kinase (ask), une aspartate-semialdéhyde déshydrogénase (asd) et une dihydrodipicolinate réductase (dapB). D'autres polypeptides codent ask, asd, dapB et diaminopimélate déshydrogénase (ddh); ask, asd, dapB, ddh et un polypeptide ORF2, et ask, asd dapB, ddh, ORF2 et un diaminopimélate décarboxylase (lysA). L'invention concerne également des méthodes d'élaboration et d'utilisation des polynucléotides et des méthodes d'augmentation de la production de L-lysine.

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