Polynucleotide participating in rheumatoid arthritis and...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/09 (2006.01) A61K 48/00 (2006.01) A61P 29/00 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2557368

Analysis is made on the DNA methylation of the region (including a promoter region) upstream from the initiation point of a rheumatoid arthritis- associated gene DR3 in human genome. As a result, it is found out that an allele-specific methylation occurs in a CpG sequence located about -380 to - 180 bp upstream from initiation point (ATG) of the gene DR3. It is further found out that the CpG sequences downstream therefrom of the genes DR3 originating in healthy subjects are all in the unmethylated state, while methylated and unmethylated sequences are both observed in the genes DR3 originating in RA patients.

On effectue une analyse sur la méthylation de l'ADN de la région (comprenant une région promoteur) en amont du point d'initiation d'un gène DR3 associé à a polyarthrite rhumatoïde dans le génome humain. En conséquence, on a trouvé qu'une méthylation spécifique à un allèle a lieu dans une séquence CpG située à environ -380 à -180 paires de bases en amont du point d'initiation (ATG) du gène DR3. On a en plus trouvé que les séquences CpG en aval de celle-ci des gènes DR3 provenant de sujets en bonne santé sont toutes à l'état non méthylé, alors qu'à la fois des séquences méthylées et non méthylées sont observées dans les gènes DR3 provenant de patients souffrant de polyarthrite rhumatoïde.

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