Polynucleotide sequence variants

Details Polynucleotide sequence variants Polynucleotide sequence variants

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2502923

We describe here an in vitro method of redistributing sequence variations between non-identical polynucleotide sequences, by making a heteroduplex polynucleotide from two non-identical polynucleotides; introducing a nick in one strand at or near a base pair mismatch site; remvoing mismatched base(s) from the mismatch site where the nick occurred; and using the opposite strand as template to replace the removed base(s) with bases that complement base(s) in the first strand. By this method, information is transferred from one strand to the other at sites of mismatch.

L'invention concerne un procédé in vitro de redistribution des variations d'une séquence entre des séquences polynucléotidiques non identiques. Ce procédé consiste à produire un polynucléotide hétéroduplex à partir de deux polynucléotides non identiques; à introduire une coupure dans un brin au niveau ou à proximité d'un site de mésappariement de paires; à retirer la/les base(s) de mésappariement du site de mésappariement où la coupure simple brin apparait; et à utiliser le brin opposé comme matrice pour remplacer la/les base(s) retirée(s) par les bases qui complètent la/les base(s) du premier brin. Ce procédé permet de transférer des informations d'un brin à l'autre au niveau de sites de mésappariement.

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