C - Chemistry – Metallurgy – 40 – B
Patent
C - Chemistry, Metallurgy
40
B
C40B 40/06 (2006.01) C07H 21/00 (2006.01) C12M 1/40 (2006.01) C12N 15/09 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01) C40B 50/06 (2006.01) C40B 50/14 (2006.01) G01N 33/50 (2006.01) G01N 35/10 (2006.01) G06F 19/00 (2006.01)
Patent
CA 2558749
Methods of improving the kinetics of bimolecular interactions where reactants are present in low concentrations are provided. Methods of pre-amplifying one or more oligonucleotides using high concentration universal primers are provided. Methods of improving the error rate in oligonucleotide and/or polynucleotide syntheses are also provided. Methods for sequence optimization and oligonucleotides design are further provided.
L'invention concerne des procédés pour améliorer la cinétique des interactions moléculaires lorsque les réactifs sont présents à faibles concentrations. Elle concerne des procédés pour pré-amplifier un ou plusieurs oligonucléotides utilisant des amorces universelles haute concentration, ainsi que des procédés pour réduire le taux d'erreurs pendant la synthèse d'oligonucléotides et/ou de polynucléotides, ainsi que des procédés d'optimisation de séquences et de conceptions d'oligonucléotides.
Church George M.
Tian Jingdong
President And Fellows Of Harvard College
Smart & Biggar
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