Polynucleotides and chimaera polypeptides for releasing a...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/62 (2006.01)

Patent

CA 2718868

The invention relates to a chimaera polypeptide that comprises a plurality of polypeptide domains and that can be released in association with membrane vesicles and in particular exosomes. The invention also relates to a polypeptide comprising one of said domains for releasing a peptide or polypeptide of interest in association with membrane vesicles and in particular exosomes. The invention also relates to the use of the polypeptides of the invention and of polynucleotides coding for said polypeptides for producing immunogenic compositions containing exosomes or DNA or for screening proteic interactions. The invention also relates to the implementation of the properties of exosomes including a polypeptide of the invention, and of immunogenic compositions of the invention for preventing and/or treating an infection due to a pathogen agent, a pathogen organism, a tumoral antigen or a cytoplasmic antigen, in particular for eliciting or promoting in vivo in a host, particularly a human or non-human mammal or a bird, a humoral and/or cellular response against a virus, a bacteria, a parasite or a tumour.

La présente invention a pour objet un polypeptide chimérique comprenant plusieurs domaines polypeptidiques, capable d'être sécrété en association avec des vésicules membranaires et en particulier des exosomes. La présente invention a également pour objet un polypeptide constitué par l'un de ces domaines permettant la sécrétion d'un peptide ou polypeptide d'intérêt en association avec des vésicules membranaire et en particulier des exosomes. L'invention concerne également l'utilisation des polypeptides de l'invention et des polynucléotides codant pour ces polypeptides pour la production de compositions immunogènes à base d'exosomes ou à base d'ADN ou pour le criblage d'interactions protéiques. La présente invention concerne également la mise en oeuvre des propriétés d'exosomes comportant un polypeptide de l'invention et de compositions immunogènes de l'invention pour la prophylaxie et/ou le traitement d'une infection par un agent pathogène, un organisme pathogène, un antigène tumoral ou un antigène cytoplasmique, en particulier pour éliciter ou favoriser in vivo, chez un hôte, en particulier chez un mammifère humain ou non humain ou un oiseau, une réponse humorale et/ou cellulaire contre un virus, une bactérie, un parasite ou une tumeur.

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