Polynucleotides encoding a beta-glucosidase and uses thereof

Details Polynucleotides encoding a beta-glucosidase and uses thereof Polynucleotides encoding a beta-glucosidase and uses thereof

C - Chemistry, Metallurgy

12

N

C12N 9/24 (2006.01) C07K 14/415 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2475572

Disclosed are polynucleotides that encode a beta-glucosidase belonging to the plant protein class of family 1 glycoside hydrolases. Furthermore described are recombinant nucleic molecules and vectors containing these polynucleotides and host cells, preferably plant cells that are genetically engineered with these polynucleotides. In addition, transgenic plants are disclosed which show an increased activity of the beta-glucosidase described, preferably leading to an improved resistance against plant pathogens. In another aspect, the present application discloses screening methods for identifying a substrate for said betaglucosidase and methods for the production of plant protection compositions from such substrates or hydrolysis products thereof. Furthermore, methods and uses are disclosed which apply the polynucleotides encoding the beta-glucosidase described in order to improve resistance in plants against plant pathogens.

L'invention concerne des polynucléotides codant une bêta-glucosidase appartenant à la classe de protéine végétale de la famille 1 des glycoside-hydrolases. De plus, l'invention concerne des molécules nucléiques recombinantes et des vecteurs renfermant ces polynucléotides et des cellules hôtes, de préférence, des cellules végétales, modifiées génétiquement au moyen de ces polynucléotides. L'invention concerne également des plantes transgéniques présentant une activité accrue de la bêta-glucosidase selon l'invention, de préférence, entraînant une résistance améliorée contre des pathogènes végétaux. Dans un autre mode de réalisation, l'invention concerne des procédés de criblage permettant d'identifier un substrat pour ladite bêta-glucosidase et des procédés de production de compositions de protection des végétaux issues de tels substrats ou des produits d'hydrolyse de ceux-ci. L'invention concerne enfin des procédés et des utilisations mettant en oeuvre les polynucléotides codant la bêta-glucosidase selon l'invention, aux fins d'amélioration de la résistance chez des végétaux contre des pathogènes végétaux.

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