Polynucleotides for the detection and quantification of...

C - Chemistry – Metallurgy – 07 – H

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C07H 21/04 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 33/576 (2006.01)

Patent

CA 2484507

The present invention provides polynucleotides that can specifically hybridise to Hepatitis B virus (HBV) nucleic acids from all genotypes. These polynucleotides can be used in genotype-independent detection and quantitation of HBV nucleic acids. For example, the polynucleotides can be used as primers and / or probes in amplification-based assays for either end-point detection or real-time monitoring of HBV nucleic acids in a test sample. The polynucleotides can additionally be provided as part of a kit for the detection and quantitation of HBV nucleic acids.

L'invention concerne des polynucléotides capables d'hybridation spécifique avec les acides nucléiques du virus de l'hépatite B de tous les génotypes. Ces polynucléotides peuvent être utilisés pour la détection et la quantification des acides nucléiques en question, indépendamment du génotype. Par exemple, on peut les utiliser comme amorces et/ou sondes dans des essais à base d'amplification, aux fins de détection d'extrémité ou de contrôle en temps réel des acides nucléiques considérés sur un échantillon d'essai. Enfin, ils peuvent être fournis à titre additionnel dans le cadre d'un kit pour la détection et la quantification de ces acides nucléiques.

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