Polynucleotides for the detection of escherichia coli

Details Polynucleotides for the detection of escherichia coli Polynucleotides for the detection of escherichia coli

C - Chemistry, Metallurgy

12

N

C12N 15/31 (2006.01) C07H 21/00 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2569085

Polynucleotide primers and probes for the specific amplification and detection of E. coli in samples are provided. The polynucleotide primers and probes are targeted to the E. coli prpP and/or ykgM genes and can detect a wide range of E. coli strains. The primers and probes can be used in real time diagnostic assays for rapid detection of E. coli in a variety of situations and are capable of distinguishing E. coli from Shigella spp. Kits comprising the primers and probes are also provided.

L'invention concerne des amorces et des sondes polynucléotidiques pour l'amplification et la détection spécifiques d'E. coli dans des échantillons. Ces amorces et ces sondes polynucléotidiques sont ciblées sur les gènes d'E. coli prpP et/ouykgM et permettent de détecter une multitude de souches d'E. coli. Lesdites amorces et sondes peuvent être utilisées dans des méthodes diagnostiques en temps réel en vue d'une détection rapide d'E. coli dans diverses situations, et permettent de distinguer E. coli de Shigella spp. L'invention concerne également des trousses comprenant ces amorces et ces sondes.

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