Polynucleotides for the detection of escherichia coli o157

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/31 (2006.01) C07H 21/00 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2558041

Polynucleotide primers and probes for the amplification and detection of E. coli O157 in a test sample are provided. The primers and probes can be used in real time diagnostic assays for rapid detection of E. coli O157 in a variety of situations, including clinical samples, microbiological pure cultures, food, and environmental and pharmaceutical quality control processes. Kits comprising the primers and probes are also provided.

La présente invention concerne des amorces et des sondes polynucléotidiques permettant l'amplification et la détection de E. coli O157 dans un échantillon d'essai. Les amorces et les sondes décrites dans cette invention peuvent être utilisées dans des dosages diagnostiques en temps réel pour permettre une détection rapide de E. coli O157 dans une large gamme de situations, y compris des échantillons cliniques, des cultures pures microbiologiques, des aliments, ainsi que dans des processus visant à contrôler la qualité à des fins environnementales et pharmaceutiques. La présente invention concerne également des trousses comprenant des amorces et des sondes.

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